The cloning revolution: DNA cloning and biochemistry
نویسندگان
چکیده
منابع مشابه
An in silico DNA cloning experiment for the biochemistry laboratory.
This laboratory exercise introduces students to concepts in recombinant DNA technology while accommodating a major semester project in protein purification, structure, and function in a biochemistry laboratory for junior- and senior-level undergraduate students. It is also suitable for forensic science courses focused in DNA biology and advanced high school biology classes. Students begin by ex...
متن کاملCloning and Religious Challenges
Genetic Engineering has conquered different arenas and life's various forms and caused drastic changes in them. Today, with the help of biotechnology and genetic engineering, mankind has reached high summits. One such summit is the decoding of human constituting particle which is the source of physical, behavioral features as well as intellectual power. This is nothing but the gene. Today man h...
متن کاملNetwork cloning using DNA barcodes
The ability to measure or manipulate network connectivity is the main challenge in the field of connectomics. Recently, a set of approaches has been developed that takes advantage of next generation DNA sequencing to scan connections between neurons into a set of DNA barcodes. Individual DNA sequences called markers represent single neurons, while pairs of markers, called barcodes contain infor...
متن کاملDNA cloning without restriction enzyme and ligase.
One common problem in using the traditional DNA cloning procedure is that suitable natural restriction sites are often unavailable for a given task. Creating new restriction sites is often time consuming. Here, I describe a simple technique of producing "customized cohesive ends" by a combination of PCR primer design and lambda exonuclease digestion. These complementary cohesive ends can form h...
متن کاملCloning and Expression of Bst DNA Polymerase I Gene in E. coli BL21
خلاصه DNA پلیمرازها علاوه بر کاربردشان در همسانه سازی و تصحیح ، در انواع تکنیک های ملکولی مانند تکثیر DNA ، جهش های نقطه ای ، توالی یابی DNA ، انواع مختلف PCR ، LAMP و ...اهمیت دارند. پس از کشف PCR تلاش هایی مبنی بر تشخیص و جداسازی آنزیم های مقاوم به دماهای بالا که توانایی تکثیر موثر DNA در دماهای بالا انجام گرفت. در این پژوهش ، سویه Geobacillus stearothermophilus strain 10 به منظور...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Biochemist
سال: 2007
ISSN: 0954-982X,1740-1194
DOI: 10.1042/bio02905012