The amino acid sequence of Acanthamoeba profilin.
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چکیده
منابع مشابه
The amino acid sequence of Acanthamoeba profilin.
The complete amino acid sequence of Acanthamoeba profilin was determined by aligning tryptic, chymotryptic, thermolysin, and Staphylococcus aureus V8 protease peptides together with the partial NH2-terminal sequences of the tryptophan-cleavage products. Acanthamoeba profilin contains 125 amino acid residues, is NH2-terminally blocked, and has trimethyllysine at position 103. At five positions i...
متن کاملThe primary structure of the basic isoform of Acanthamoeba profilin.
Acanthamoeba profilin-II [Kaiser, D.A., Sato, M., Ebert, R. F. and Pollard, T.D. (1986) J. Cell. Biol. 102, 221-226] was digested with trypsin or cleaved by 2-(2-nitrophenylsulphenyl)-3-methyl-3-bromoindolenine. The tryptic peptides were purified by reversed-phase-high-performance liquid chromatography and completely sequenced using automated gas-phase sequence analysis. The complete profilin-I...
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Acanthamoeba profilin was cross-linked to actin via a zero-length isopeptide bond using carbodiimide. The covalently linked 1:1 complex was purified and treated with cyanogen bromide. This cleaves actin into small cyanogen bromide (CNBr) peptides and leaves the profilin intact owing to its lack of methionine. Profilin with one covalently attached actin CNBr peptide was purified by gel filtratio...
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We investigated the mechanical properties of two abundant, cytoplasmic proteins from Acanthamoeba, profilin and actin, and found that while both profilin and nonfilamentous actin alone behaved as solids, mixtures of the two proteins were viscoelastic liquids. When allowed to equilibrate, profilin formed a viscoelastic solid with mechanical properties similar to filamentous and nonfilamentous ac...
متن کاملThe amino acid sequence of an extracellular nuclease of Staphylococcus aureus. 3. Complete amino acid sequence.
In a previous paper data were presented that permitted the linear arrangement of five fragments produced by cleavage of an extracellular nuclease of Staphylococcus aureus with cyanogen bromide (1). The amino acid sequences of the tryptic peptides prepared from these fragments, together with the partial sequences of chymotryptic peptides isolated from digests of the intact nuclease, have also be...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1985
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(20)71174-9