Surface Corrosion from Implant–Abutment Couplings with Different Connection Designs Influences Osteoblasts’ Function: A Novel Technique

The improved peri-implant bone response demonstrated when utilizing the platform-switching concept may result from reduced levels of metal ions released implant–abutment surfaces to surrounding tissues. These corrosion products play a major role in crestal remodeling around dental implants. This study evaluated effect different couplings (platform-matched vs. platform-switched) on osteoblasts’ function. Titanium alloy and cobalt–chrome abutments were coupled with titanium cylinders, forming either platform-switched or platform-matched groups, incubated human osteoblast cultures novel direct-exposure technique. Viability was over 21 days using Alamar Blue assay. Apoptosis measured after 24 h flow cytometry. expression genes related resorption analysed real-time quantitative polymerase chain reaction Cell viability day 4 (p < 0.05), higher rates early apoptosis 0.05) compared controls. groups 0.05). tested genes’ up-regulated 1 3 exposure upregulation more pronounced Exposure osteoblasts induced adverse biological responses, which couplings. reactions might be increased amounts couplings, highlighting possible mediation loss