Stem Cell Derived Retinal Pigment Epithelium: The Role of Pigmentation as Maturation Marker and Gene Expression Profile Comparison with Human Endogenous Retinal Pigment Epithelium.
نویسندگان
چکیده
منابع مشابه
Gene expression profile of native human retinal pigment epithelium.
PURPOSE To generate a profile of genes expressed in the native human retinal pigment epithelium and identify candidate genes for retinal and macular diseases. METHODS Two cDNA libraries (one amplified, the other unamplified) were constructed using RNA isolated from native human RPE sheets. The sequence from the 5' end was obtained for randomly selected clones from the two libraries. Of these,...
متن کاملinvestigation of melanogenic factors gene expression in human adult and neonate retinal pigment epithelium cell cultures
retinal pigment epithelium is responsible for maintaining the structural integrity of the retina by an efficient defense against free radicals, photo-oxidative exposure and light energy. for this purpose the main rpe line of defense is melanosomes. melanin content of retinal pigment epithelium cells in adults and neonates reveals remarkable variations. in the current study we compared melanogen...
متن کاملRetinal Pigment Epithelium Derived Stem Cells
Retinal pigment epithelial (RPE) stem cells offer a theoretical treatment strategy for a wide variety of retinal disorders including age-related macular degeneration (AMD) and retinal dystrophies. There are, however, a number of hurdles that will have to be overcome before a consistently reliable and successful therapy can be offered to patients. These include expansion of sufficient numbers of...
متن کاملHistochemical study of retinal photoreceptors development during pre- and postnatal period and their association with retinal pigment epithelium
Objective(s):The aim of this study was to evaluate distribution and changes of glycoconjugates of retinal photoreceptors during both pre- and postnatal development. Materials and Methods: Tissue sections from days 15 to 20 of Wistar rat embryos and 1 to 12 postnatal days of rat newborns including developing eye were prepared for lectinhistochemistry technique. Horseradish peroxidase (HRP)-label...
متن کاملDetection of Retinal Pigment Epithelium-Specific Antibody in iPSC-Derived Retinal Pigment Epithelium Transplantation Models
Antibody-mediated rejection is characterized by donor-specific antibody produced by B cells. However, to our knowledge, B cell invasion and antibody in the inflamed retina after transplantation of retinal pigment epithelial (RPE) cells has not been reported. To determine if RPE transplantation could be performed using allografts, we established in vivo immune rejection models with induced pluri...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Stem Cell Reviews and Reports
سال: 2017
ISSN: 1550-8943,1558-6804
DOI: 10.1007/s12015-017-9754-0