Specific detection of fungal pathogens by 18S rRNA gene PCR in microbial keratitis

Specific detection of fungal pathogens by 18S rRNA gene PCR in microbial keratitis

BACKGROUND The sensitivity and specificity of 18S rRNA polymerase chain reaction (PCR) in the detection of fungal aetiology of microbial keratitis was determined in thirty patients with clinical diagnosis of microbial keratitis. METHODS Corneal scrapings from patients were used for Gram stain, culture and PCR analysis. PCR was performed with primer pairs targeted to the 18S rRNA gene. The res...

Author ' s response to reviews Title : Specific detection of fungal pathogens by 18 S rRNA gene PCR in microbial

Detection of fungi by conventional methods and semi-nested PCR in patients with presumed fungal keratitis

Background and Purpose: Fungal keratitis is a suppurative, ulcerative, and sight-threatening infection of the cornea that sometimes leads to blindness. The aims of this study were: recuperating facilities for laboratory diagnosis, determining the causative microorganisms, and comparing conventional laboratory diagnostic tools and semi-nested PCR. Materials and Methods: Sampling was conducted in...

Detection of fungal 18S rRNA sequences in conjunction with marine nematode 18S rRNA amplicons

Free-living nematodes constitute an important component of estuarine and marine benthic ecosystems. Some marine and soil nematodes are known to harbor microbes, including symbiotic bacteria and fungi, in their external cuticle as well as internally. While assessing diversity of marine nematodes from southwest England using molecular approaches, we found evidence of coamplification of fungal 18S...

PCR Detection of Microbial Pathogens

This book covers general challenges of introducing primarily noncommercial PCRs and specific procedures into the laboratory, including sample treatment, extraction protocols, quality and quality assurance, and internal and external laboratory processes. The chapters on specific pathogens illustrate principles that could be applied in many diagnostic laboratories. The editor’s preface to this bo...