Sorting Out Bacterial Viability with Optical Tweezers
نویسندگان
چکیده
منابع مشابه
Microfluidic sorting of arbitrary cells with dynamic optical tweezers.
Optical gradient forces generated by fast steerable optical tweezers are highly effective for sorting small populations of cells in a lab-on-a-chip environment. The presented system can sort a broad range of different biological specimens by an automated optimisation of the tweezer path and velocity profile. The optimal grab positions for subsequent trap and cell displacements are estimated fro...
متن کاملOPTiCAL TwEEzERS CALibRATiON AND bACTERiAL ADHESiON
EXECUTiVE SUMMARY In this project, we will be measuring the forces of the binding of bacteria. We will be working with Lactobacillus acidophilus NCFM, Lactobacillus galli narum 33199, Lactobacillus ruteri 23272, and Bifidobacterium. These bacteria are lactic acid bacteria and are used as probiotics. We also reviewed literature on the bacterial cell wall constituents and the properties of adhes...
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Optical trapping is a powerful tool for the micromanipulation of living cells--especially bacteria--but photodamage induced by the laser beam can adversely affect viability. We have explored optical trapping conditions in the near infrared (840-930 nm) that preserve the viability of E. coli, as measured by gene expression of green fluorescent protein. We have found that time-sharing the optical...
متن کاملNanoscopy of bacterial cells immobilized by holographic optical tweezers
Imaging non-adherent cells by super-resolution far-field fluorescence microscopy is currently not possible because of their rapid movement while in suspension. Holographic optical tweezers (HOTs) enable the ability to freely control the number and position of optical traps, thus facilitating the unrestricted manipulation of cells in a volume around the focal plane. Here we show that immobilizin...
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Force-extension (F-x) relationships were measured for single molecules of DNA under a variety of buffer conditions, using an optical trapping interferometer modified to incorporate feedback control. One end of a single DNA molecule was fixed to a coverglass surface by means of a stalled RNA polymerase complex. The other end was linked to a microscopic bead, which was captured and held in an opt...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2000
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.182.19.5551-5555.2000