Sexual reproduction in two mixed stands of coastal and interior Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) in Germany

Abstract Adult individuals and seeds of two mixed stands coastal interior Douglas-fir ( Pseudotsuga menziesii (Mirb.) Franco) were analysed for genetic differentiation between the varieties evidence intervarietal pollination. Clear discrimination was observed based on multilocus evaluation nine microsatellite markers using other known variety composition comparison. Analysis pollination distances showed that 80% pollinations took place within a distance about 44–55 m. stand structure clearly separated areas mainly or both stands. Together with short this led to an apparent dominance intravarietal pollinations. However, analysis partners trees growing near border specific areas, does not indicate existence reproductive barriers in Therefore, commercial seed harvesting should be avoided if production lots pure is intended.