Selection of oocytes for in vitro maturation by brilliant cresyl blue staining: a study using the mouse model
نویسندگان
چکیده
منابع مشابه
Selection of Ovine Oocytes by Brilliant Cresyl Blue Staining
Sheep oocytes derived from the ovaries collected from the slaughterhouse are often used for research on in vitro embryo production, animal cloning, transgenesis, embryonic stem cells, and other embryo biotechnology aspects. Improving the in vitro culture efficiency of oocytes can provide more materials for similar studies. Generally, determination of oocyte quality is mostly based on the layers...
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this study investigates the cohesive devices used in the textbook of english for the students of psychology. the research questions and hypotheses in the present study are based on what frequency and distribution of grammatical and lexical cohesive devices are. then, to answer the questions all grammatical and lexical cohesive devices in reading comprehension passages from 6 units of 21units th...
Brilliant Cresyl Blue in Sap of Nitella
It has been shown by several investigators I that when the pl-I value of the external dye solutions is increased, the rate of accumulation of a basic dye in the cell sap is accelerated. Two different explanations for this have been given. Some have accounted for it on the basis that the basic dye enters the cell in the form of a dye hydrate and combines with the substances in the cell; and that...
متن کاملP-100: Improved Efficiency of Somatic Cell Nuclear Transfer in Sheep by Persecution of Recipient Oocytes with Brilliant Cresyl Blue Staining
Background: The most important factor affecting the efficiency of somatic cell nuclear transfer (SCNT) is the initial quality of the oocyte. Since the abattoir-derived oocytes are heterogeneous in quality, selection of cohort fully grown/competent oocytes is necessary for proper development of SCNT embryos. Materials and Methods: Glucose-6-phosphate dehydrogenase (G6PDH) is actively expressed i...
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هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
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ژورنال
عنوان ژورنال: Cell Research
سال: 2007
ISSN: 1001-0602,1748-7838
DOI: 10.1038/cr.2007.66