Screening of Antibiotics Using Mammalian Cells

Cytotoxicity of several marketed antibiotics on mammalian cells in culture.

The cytotoxicity and biochemical effects of several marketed antibiotics on four mammalian cell lines were determined. Several metabolites of clindamycin and several clinically useful anticancer drugs were also included in this study. The four cell lines were mouse leukemia L1210, human oral carcinoma KB, human acute myelogenous leukemia RPMI 6410, and human lymphocyte RPMI 1788. At concentrati...

Screening of antibiotics preferentially active against ras oncogene-expressed cells.

During the course of in vitro screening of agents which are preferentially active against ras oncogene-expressed cells, a new anthracycline (identified as 2-demethylsteffimycin D) and a heptaene (possibly a new member of partricins) were isolated from microbial fermentation broths. Among known compounds tested, 5-fluorouracil, 5-fluorodeoxyuridine and oxanosine showed high selectivity towards r...

MISSION esiRNA for RNAi screening in mammalian cells.

RNA interference (RNAi) is a basic cellular mechanism for the control of gene expression. RNAi is induced by short double-stranded RNAs also known as small interfering RNAs (siRNAs). The short double-stranded RNAs originate from longer double stranded precursors by the activity of Dicer, a protein of the RNase III family of endonucleases. The resulting fragments are components of the RNA-induce...

screening of bacillus species with potentials of antibiotics production

Cells O Antibody Screening Cells: A novel Approach to Antibody Screening

ABSTRACT        Background and Objectives: Blood transfusion may induce some adverse effects on receivers. Some methods such as antibody screening and cross matching have been suggested to reduce the risk of transfusion complications. However, these methods require commercial antibody screening kits that may also need special equipment. The aim of this study was t...