Screening for in vivo protein-protein interactions.
نویسندگان
چکیده
منابع مشابه
Discovering Domains Mediating Protein Interactions
Background: Protein-protein interactions do not provide any direct information regarding the domains within the proteins that mediate the interactions. The majority of proteins are multi domain proteins and the interaction between them is often defined by the pairs of their domains. Most of the former studies focus only on interacting domain pairs. However they do not consider the in...
متن کاملIn Vivo Imaging of Protein-Protein Interactions
Protein-protein interaction (PPI) plays a pivotal role in a wide variety of cellular events and physiological functions, such as enzymatic activity, signal transduction, immunological recognition, DNA repair/replication, among others (Valdar and Thornton, 2001). In addition, biological events that regulate proliferation, differentiation, and inflammation are also commonly mediated through PPI (...
متن کاملImaging Protein-protein Interactions in vivo
Protein-protein interactions are a hallmark of all essential cellular processes. However, many of these interactions are transient, or energetically weak, preventing their identification and analysis through traditional biochemical methods such as co-immunoprecipitation. In this regard, the genetically encodable fluorescent proteins (GFP, RFP, etc.) and their associated overlapping fluorescence...
متن کاملTechniques for the Analysis of Protein-Protein Interactions in Vivo.
Identifying key players and their interactions is fundamental for understanding biochemical mechanisms at the molecular level. The ever-increasing number of alternative ways to detect protein-protein interactions (PPIs) speaks volumes about the creativity of scientists in hunting for the optimal technique. PPIs derived from single experiments or high-throughput screens enable the decoding of bi...
متن کاملPooled screening for anti-proliferative inhibitors of protein-protein interactions
Protein-protein interactions (PPIs) are emerging as a promising new class of drug targets. Here, we present a novel high-throughput approach to screen inhibitors of PPIs in cells. We designed a library of 50,000 human peptide-binding motifs and used a pooled lentiviral system to express them intracellularly and screen for their effects on cell proliferation. We thereby identified inhibitors tha...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1993
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.90.3.933