Revealing large metagenomic regions through long DNA fragment hybridization capture

Revealing large metagenomic regions through long DNA fragment hybridization capture

BACKGROUND High-throughput DNA sequencing technologies have revolutionized genomic analysis, including the de novo assembly of whole genomes from single organisms or metagenomic samples. However, due to the limited capacity of short-read sequence data to assemble complex or low coverage regions, genomes are typically fragmented, leading to draft genomes with numerous underexplored large genomic...

Magnetic capture hybridization of Batrachochytrium dendrobatidis genomic DNA.

We hybridized biotinylated probes that anneal at multiple locations throughout the Batrachochytrium dendrobatidis (Bd) genome to selectively capture Bd genomic DNA (gDNA) by binding the probe-gDNA complex to streptavidin coated magnetic beads. We then whole genome amplified the captured gDNA. This method extends the usefulness of field-collected swabs for downstream PCR-based genomic applications.

Comparative genomic hybridization using DNA from laser capture microdissected tissue.

Comparative genomic hybridization (CGH) is a powerful screening technique that can identify regions of gain and loss within the whole genome in a single experiment. The combination of laser capture microdissection, whole-genome amplification, and CGH permits genomic screening with high specificity and sensitivity. This complement of techniques has enabled analysis of focal regions and subpopula...

Hybridization Capture Using RAD Probes

Selective enrichment of a large size genomic DNA fragment by affinity capture: an approach for genome mapping.

A method to enrich large size DNA fragments obtained by digestion with rare cutting restriction endonucleases was developed and applied for the isolation of a 150 kb SfiI fragment containing the beta-globin gene cluster. The digested DNA is rendered single stranded at the ends by diffusing a strand specific exonuclease into an agarose plug containing DNA. The plug is melted and solution hybridi...