Replication of Orgyia pseudotsugata Baculovirus DNA: lef-2 and ie-1 Are Essential and ie-2, p34, and Op-iap Are Stimulatory Genes
نویسندگان
چکیده
منابع مشابه
2. IE & IEs
PROTO-INDO-EUROPEAN is the traditional name given to the ancestor language of the Indo-European family that is spread from Iceland to Chinese Turkestan and from Scandinavia to the Near East. A PROTO-LANGUAGE (Gk. prõtos ‘first’) refers to the earliest form of a language family presupposed by all of its descendants. There will forever be major gaps in the reconstruction of proto-languages, but a...
متن کاملBaculovirus replication factor LEF-1 is a DNA primase.
The baculovirus replication factors LEF-1 and LEF-2 of the Autographa californica multinucleocapsid nucleopolyhedrovirus were overexpressed as fusions containing a hemagglutinin (HA) epitope and a HIS(6) tag using recombinant baculoviruses. LEF-1 was purified to near homogeneity and found to have primase activity in an indirect assay employing Escherichia coli DNA polymerase I (Klenow enzyme) a...
متن کاملIn vivo and in vitro analysis of baculovirus ie-2 mutants.
Upon transient expression in cell culture, the ie-2 gene of Autographa californica nuclear polyhedrosis virus (AcMNPV) displays three functions: trans activation of viral promoters, direct or indirect stimulation of virus origin-specific DNA replication, and arrest of the cell cycle. The ability of IE2 to trans stimulate DNA replication and coupled late gene expression is observed in a cell lin...
متن کاملThe roles of eighteen baculovirus late expression factor genes in transcription and DNA replication.
A set of 18 plasmid subclones of the Autographa californica nuclear polyhedrosis virus genome supports expression from a late viral promoter in transient expression assays (J. W. Todd, A. L. Passarelli, and L. K. Miller, J. Virol. 69:968-974, 1995). Using this set of plasmids, we have assigned a role for each of the 18 genes required for optimal late gene expression with respect to its involvem...
متن کاملThe c-IAP-1 and c-IAP-2 proteins are direct inhibitors of specific caspases.
The inhibitor of apoptosis (IAP) family of proteins are highly conserved through evolution. However, the mechanisms by which these proteins interfere with apoptotic cell death have been enigmatic. Recently, we showed that one of the human IAP family proteins, XIAP, can bind to and potently inhibit specific cell death proteases (caspases) that function in the distal portions of the proteolytic c...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Virology
سال: 1995
ISSN: 0042-6822
DOI: 10.1006/viro.1995.1523