Real-time monitoring of caspase cascade activation in living cells

Spontaneous Mutation: Real-Time in Living Cells

A new study provides the first direct visualization of DNA replication errors as they become mutations in living cells. After decades of post-hoc inference of mutation rates and mechanisms, a new method opens the possibility of cell-, tissue- and cancer-clone-specific mutation-rate detection and real-time visualization of transient hypermutable states in situ.

Watching real-time endocytosis in living cells

The precise sequence of events promoting clathrin-coated vesicle assembly is still debated. In this issue, Kadlecova et al. (2017. J. Cell Biol. https://doi.org/10.1083/jcb.201608071) test structural models using quantitative microscopy in living cells to investigate the hierarchy and temporal importance of molecular events required for clathrin-coated pit initiation.

Dynamic Monitoring of Receptor Tyrosine Kinase Activation in Living Cells

Fluorescence Cross-Correlation Spectroscopy for Real-Time Monitoring of Exogenous DNA Behavior in Living Cells

Many kind of DNA transfection techniques have been developed and widely used in the field of biochemical assay (Boussif et al., 1995, Chu et al., 1987, Felgner et al., 1987). Since recent development of fluorescent proteins such as green fluorescent protein (GFP), its variants and bio sensors, DNA transfection techniques play a greater role in the field of cell biology especially in bioimaging ...

Real-time detection of caspase-2 activation in a single living HeLa cell during cisplatin-induced apoptosis.

Caspase-2 is important for the mitochondrial apoptotic pathway, however, the mechanism by which caspase-2 executes apoptosis remains obscure. We carry out the first measurements of the dynamics of caspase-2 activation in a single living cell by a FRET (fluorescence resonance energy transfer) probe. Two FRET probes are constructed that each encoded a CRS (caspase-2 or caspase-3 recognition site)...