Quantitative real-time PCR for the clinical detection of Helicobacter pylori
نویسندگان
چکیده
منابع مشابه
Real-time quantitative PCR for detection of Helicobacter pylori.
Helicobacter pylori is one of the most common chronic infections in humans, in whom it is a key etiological factor in peptic ulcer disease, gastric mucosa-associated lymphoid tissue lymphoma, and gastric adenocarcinoma. Humans are the bacterium's only host. Here we report the development of a real-time quantitative (Q) PCR-based assay to measure ureC gene copy number to detect H. pylori, based ...
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Accurate diagnosis of Helicobacter pylori infection is very important in both clinical practice and research. We evaluated the sensitivity of real-time PCR (RT-PCR) for the detection and quantification of Helicobacter pylori using DNA from 91 human gastric biopsy samples divided into three groups: 46 biopsies from untreated patients who according to the references methods were considered H. pyl...
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Accurate diagnosis of Helicobacter pylori infection is important in both clinical practice and clinical research. Molecular methods are highly specific and sensitive, and various PCR-based tests have been developed to detect H. pylori in gastric biopsy specimens. We optimized a sensitive and specific quantitative SYBR Green I real-time PCR assay for detection of H. pylori based on amplification...
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We have investigated in the present study that H. pylori as causative agent of GC remain controversial; therefore, we planned to study the presence of H. pylori infection in patients with GC. We also investigated the presence of H. pylori infection in patients with peptic ulcer disease (PUD) and non-ulcer dyspepsia (NUD). The subjects of this study comprised of 64 patients (age 24 to 71 yrs, 36...
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ژورنال
عنوان ژورنال: Genetics and Molecular Biology
سال: 2007
ISSN: 1415-4757
DOI: 10.1590/s1415-47572007000300022