Quantitative Determination of Ascorbic Acid in Herbal Medicinal Products by HPLC

Ascorbic acid (vitamin C) is involved in many vital biochemical processes the human body. Rose hips are a natural source of ascorbic acid. used decoctions and vitamin herbal teas. Russian compendial methods for quantitative determination have certain limitations precluding its objective measurement drugs, drug preparations, medicinal products. The aim study was to develop an analytical procedure products using high performance liquid chromatography (HPLC). Materials . two products, Hips (rose hips) Vitamin Herbal Tea 2 rowan fruits), reference standards acid, rutoside, quercetin, gallic caffeic chlorogenic luteolin-7-glucoside, citric DL-malic thiamine, pyridoxine hydrochloride. authors 1260 Infinity II chromatograph with diode array detector by Agilent Inertsil ODS-3 chromatographic column (250 mm × 4.6 mm, 5 µm) GL Sciences. HPLC system operated gradient elution mode, set at 244 nm. Results. content can be determined HPLC. Adequate sample preparation conditions allow inhibiting oxidation aqueous solutions period sufficient complete testing (not less than 8 h). Conclusions. developed highly sensitive selective intended standardisation worthy inclusion monographs State Pharmacopoeia Federation. Switching should not require lowering limit 0.2%) established titration method. below this vast majority samples. However, discrepancy may explained presence dog rose (Rosa canina) species low C, which normally only as choleretics.