Purification and Properties of Spinach Leaf Debranching Enzyme
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چکیده
منابع مشابه
Purification and properties of spinach leaf debranching enzyme.
Starch debranching enzyme was purified from intact spinach (Spinacia oleracea L. cv Vital) chloroplasts and from a spinach leaf extract using affinity chromatography on Sepharose 6B-bound cycloheptaamylose (Schardinger beta-dextrin). The enzyme from both sources was homogeneous upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Spinach leaf debranching enzyme appears to consist of ...
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Cytoplasmic fructose-1,6-bisphosphatase has been purified from spinach leaves to apparent homogeneity. The enzyme is a tetramer of molecular weight about 130,000. At pH 7.5, the Km for fructose 1.6-bisphosphate was 2.5 micron, and for MgCl2 0.13 mM; the enzyme was specific for fructose 1,6-bisphosphate. Saturation with Mg2+ was achieved with lower concentrations at pH 8 than at pH 7. AMP and hi...
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Acetyl-coenzyme A (CoA) synthetase was purified 364-fold from leaves of spinach (Spinacia oleracea L.) using ammonium sulfate fractionation followed by ion exchange, dye-ligand, and gel permeation chromatography. The final specific activity was 2.77 units per milligram protein. The average M(r) value of the native enzyme was about 73,000. The Michaelis constants determined for Mg-ATP, acetate, ...
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In 1939, Hill (1) reported the fundamental discovery that isolated chloroplasts possess the ability to affect a portion of the over-all reaction of photosynthesis; namely, the photolysis of water. In the original experiments of Hill, ferric oxalate was used as the hydrogen acceptor. During the past 20 years, a variety of substances has been found to function as oxidant for the Hill reaction (2)...
متن کاملPurification and properties of glutamine synthetase from spinach leaves.
The chloroplastic glutamine synthetase of spinach leaves has been purified to homogeneity using affinity chromatography. This involves a tandem ;reactive blue A-agarose' and ;reactive red-A-agarose' as the final step in the procedure. This procedure results in a yield of 18 milligrams of pure glutamine synthetase per kilogram of starting material. The purity of our enzyme has been demonstrated ...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1984
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.74.4.856