Protochlorophyllidebdoes not occur in barley etioplasts
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چکیده
منابع مشابه
Cryopreservation of Chlorophyll Synthesis and Apoprotein Stabilization in Barley Etioplasts.
Methods for the cryopreservation of protein import and integration in pea chloroplasts and of protein import or protein synthesis in tobacco mitochondria were modified to yield enzymatically active cryopreserved etioplasts from barley (Hordeum vulgare L.). The cryoprotectants ethylene glycol and dimethy sulfoxide were about 64 and 77% effective, respectively, for the cryopreservation of etiopla...
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The etioplasts of fully dark-grown barley leaves exhibit a relatively low frequency of crystalline prolamellar bodies (ca. 16-20%). Brief red-light treatment leads to rapid disruption of all prolamellar bodies followed by a slow reformation in the subsequent dark period. When several red-light treatments are given with intervening 3-h dark periods, a marked increase in the proportion of crystal...
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A procedure permitting the isolation of large amounts of cytochrome b-559 from chloroplasts of barley is described. The isolated cytochrome b-559 has an apparent molecular weight of 120,000. The protein equivalent weight per mole of heme is 30,000. Depending on the electrophoretic conditions used, this molecule can be dissociated into components with apparent molecular weights of 31,000, 30,000...
متن کاملIn vitro synthesis of chlorophyll a in the dark triggers accumulation of chlorophyll a apoproteins in barley etioplasts.
An in vitro translation system using lysed etioplasts was developed to test if the accumulation of plastid-encoded chlorophyll a apoproteins is dependent on the de novo synthesis of chlorophyll a. The P700 apoproteins, CP47 and CP43, were not radiolabeled in pulsechase translation assays employing lysed etioplasts in the absence of added chlorophyll precursors. When chlorophyllide a plus phytyl...
متن کاملWhen does new particle formation not occur?
When does new particle formation not occur in the upper troposphere? D. R. Benson, L.-H. Young, S.-H. Lee, T. L. Campos, D. C. Rogers, and J. Jensen Kent State University, Department of Chemistry, Kent, OH, USA National Center for Atmospheric Research, Earth Observing Laboratory, Broomfield, CO, USA Received: 4 September 2007 – Accepted: 1 October 2007 – Published: 8 October 2007 Correspondence...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1999
ISSN: 0014-5793
DOI: 10.1016/s0014-5793(99)00169-6