Properties of Chitin Synthetase from Coprinus cinereus
نویسندگان
چکیده
منابع مشابه
Some characteristics of extracellular proteinases from Coprinus cinereus
Spent culture medium contained five electrophoretically distinct proteolytic activities when Coprinus c;llereus was grown in culture medium which contained protein as sole source of nitrogen. The enzymes were separated by ultrafiltration, gel filtration and ionexchange chromatography into two groups designated EL and AD fractions. The EL fraction passed through a DE-52 column with eluant, and c...
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The properties and behavior of a “microsomal” (100,000 X g particles) chitin synthetase of Mucor rouxii were investigated. The enzyme utilizes uridine diphosphate N-acetylD-glucosamine (UDP-GlcNAc) as glycosyl donor and is strongly and specifically activated by free N-acetyl-o-glucosamine (GlcNAc). A variety of GlcNAc analogues were tested as activators but were found ineffective. A small propo...
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A particulate preparation from a spheroplast lysate of Saccharomyces cartsbergensis was found to catalyze the transfer of acetylglucosamine from UDP-acetylglucosamine to’an endogenous acceptor. Uridine diphosphate is liberated in stoichiometric amounts. A divalent cation, MgZ+, Mn2+, or Co2+, is required for enzymatic activity. Acetylglucosamine stimulates the activity about 5-fold, with a K, o...
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Mushrooms, such as Coprinus cinereus, possess large families of pheromones and G-protein-coupled receptors that are sequestered at the B mating-type locus and whose function is to confer vast numbers of different mating types. This ability results from complex patterns of cognate and noncognate pheromone/receptor pairings, which potentially offer a unique insight into the molecular interaction ...
متن کاملDe novo methylation of repeated sequences in Coprinus cinereus.
We have examined the stability of duplicated DNA sequences in the sexual phase of the life cycle of the basidiomycete fungus, Coprinus cinereus. We observed premeiotic de novo methylation in haploid nuclei containing either a triplication, a tandem duplication, or an ectopic duplication. Methylation changes were not observed in unique sequences. Repeated sequences underwent methylation changes ...
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1975
ISSN: 0022-1287
DOI: 10.1099/00221287-89-1-137