منابع مشابه
Substrate-dependent proton antiport in neurotransmitter:sodium symporters
Neurotransmitter-sodium symporters (NSS), targets for psychostimulants and therapeutic drugs, have a critical role in neurotransmission. Whereas eukaryotic NSS show chloride-dependent transport, bacterial NSS feature Cl(-)-independent substrate transport. Recently we showed that mutation of an acidic residue near one of the sodium ion-binding sites in LeuT of Aquifex aeolicus or Tyt1 of Fusobac...
متن کاملNa+/Ca2+ antiport in the mammalian heart.
The cardiac Na+/Ca2+ antiporter moves 3 Na+ across the plasma membrane in exchange for a single Ca2+ moving in the opposite direction. It is the principal Ca2+ efflux mechanism in myocardial cells; however, it also contributes to Ca2+ influx under certain conditions. It is particularly abundant in the heart, but is also expressed in other tissues such as smooth and skeletal muscle, the kidney a...
متن کاملK+/H+ antiport in heart mitochondria.
Heart mitochondria depleted of endogenous divalent cations by treatment with A23187 and EDTA swell in (a) K+ acetate or (b) K+ nitrate when an uncoupler is present. These mitochondria also exchange matrix 42K+ with external K+, Na+, or Li+ in a reaction that does not require respiration and is insensitive to uncouplers. Untreated control mitochondria do not swell in either medium nor do they sh...
متن کاملCation/proton antiport systems in Escherichia coli.
The calcium/proton antiporter of Escherichia coli was characterized by measurement of the effect of divalent cations on the respiration-coupled quenching of the fluorescence of quinacrine or 9-aminoacridine in everted membrane vesicles. Energy-linked quenching of the fluorescence of aminoacridines is dependent on proton uptake into everted vesicles and provides an indirect assay for measurement...
متن کاملRole of chloride/bicarbonate antiport in the control of cytosolic pH. Cell-line differences in activity and regulation of antiport.
Sodium-linked and sodium-independent HCO3-/Cl- antiport was measured under different conditions in a number of cell lines. Transport of HCO3- was estimated from its effect on intracellular pH (pHi) measured with the fluorescent probe 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein. The associated ion fluxes were estimated from the transport of 36Cl- and 22Na+. Na+-dependent and Na+-independent H...
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ژورنال
عنوان ژورنال: Molecular Microbiology
سال: 1990
ISSN: 0950-382X,1365-2958
DOI: 10.1111/j.1365-2958.1990.tb00539.x