Oxalate determination in urine using an immobilized enzyme on sorghum vulgare seeds in a flow injection conductimetric system
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منابع مشابه
Determination of urea in serum by using naturally immobilized urease in a flow injection conductimetric system.
A flow injection method was developed, aimed at the determination of urea in human serum. The system makes use of the naturally immobilized urease present in Canavalia ensiformis DC (jack bean). A column is filled with small pieces of this bean, and the sample (50 microliters) containing urea passes through it carried by a 1% NaCl solution. On leaving the column the stream is merged with an alk...
متن کاملUrinary oxalate determination by use of immobilized oxalate oxidase in a continuous-flow system.
In this procedure, oxalate oxidase (EC 1.2.3.4) immobilized in a continuous-flow system is used to determine oxalate in urine. The hydrogen peroxide formed from oxalate is detected by use of a color reaction with peroxidase (EC 1.11.1.7), 3-methyl-2-benzothiazoline hydrazine, and N,N-dimethylalanine. However, urine contains an oxalate oxidase inhibitor, which cannot be removed by heating, ion-e...
متن کاملEnzymatic oxalate determination in urine.
1THE ACCURATE MEASUREMENT of oxalate concentration in urine presents considerable difficulties. The methods available in the literature are based upon either the direct formation of calcium oxalate precipitate and subsequent oxidimetric titration (1-3) or upon a preliminary ether extraction followed by a titrimetric procedure (4, .5). In a more elaborate method, the extraction with ether is com...
متن کاملa study on rate making and required reserves determination in reinsurance market: a simulation
reinsurance is widely recognized as an important instrument in the capital management of an insurance company as well as its risk management tool. this thesis is intended to determine premium rates for different types of reinsurance policies. also, given the fact that the reinsurance coverage of every company depends upon its reserves, so different types of reserves and the method of their calc...
Flow-injection determination of thyroxine using immobilized enzyme with tris(2,2'-bipyridyl)ruthenium(III) chemiluminescence detection.
A flow-injection method is reported for the determination of thyroxine based on its enhancement effect on the tris(2,2'-bipyridyl)ruthenium(III) chemiluminescence reaction in the presence of NADH using immobilized alcohol dehydrogenase purified from baker yeast. The limit of detection (3 sigma blank) was 5.0 x 10(-8) M with a sample throughput of 80 h(-1). The calibration graph was linear over ...
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ژورنال
عنوان ژورنال: Journal of the Brazilian Chemical Society
سال: 1997
ISSN: 0103-5053
DOI: 10.1590/s0103-50531997000100009