Opioid receptor expression in human brain and peripheral tissues using absolute quantitative real-time RT-PCR

Evaluation of GNMT Gene Expression in Prostate Cancer Tissues using Real-Time PCR

Introduction: Prostate cancer is the second most common cancer and the leading cause of cancer-related deaths worldwide. In the present study, the expression level of glycine N-methyl transferase gene (GNMT) was investigated in prostate cancer tissue. The GNMT enzyme is encoded by the GNMT gene. Increased GNMT gene expression increases the conversion of glycine to sarcosine and results in the e...

Detection and quantitation of bovine respiratory syncytial virus using real-time quantitative RT-PCR and quantitative competitive RT-PCR assays.

A single tube, fluorogenic probe-based, real-time quantitative reverse transcription-polymerase chain reaction (Q-RT-PCR) assay was developed for detection and quantitation of bovine respiratory syncytial virus (BRSV) using BioRad's iCycler iQ. Real-time Q-RT-PCR was compared with quantitative competitive RT-PCR (QC-RT-PCR) and viral titers. Viral mRNA levels were measured in BRSV-infected bovi...

Real-time quantitative RT-PCR: design, calculations, and statistics.

Meloidogyne javanica Chorismate Mutase Transcript Expression Profile Using Real-Time Quantitative RT-PCR.

A developmental expression profile of the Meloidodgyne javanica esophageal gland gene chorismate mutase-1 (Mj-cm-1) could suggest when in the lifecycle of the nematode the Mj-cm-1 product is functional. This study used real-time quantitative RT-PCR to examine the variation in Mj-cm-1 transcript levels over six timepoints in the nematode lifecycle: egg, infective second-stage juveniles (Inf-J2),...

Validation of putative housekeeping genes for gene expression studies in human hepatocellular carcinoma using real-time quantitative RT-PCR

Validation of putative housekeeping genes for gene expression studies in human hepatocellular carcinoma using real-time quantitative RT-PCR. BMC Cancer 2008, 8:350 doi:10.1186/1471-2407-8-350 Vito R Cicinnati ([email protected]) Qingli Shen ([email protected]) Georgios C Sotiropoulos ([email protected]) Arnold Radtke ([email protected]) Guido Gerken ([email protected]...