NMR Analysis of Actin Binding Protein Cofilin

Cofilin-induced cooperative conformational changes of actin subunits revealed using cofilin-actin fusion protein

To investigate cooperative conformational changes of actin filaments induced by cofilin binding, we engineered a fusion protein made of Dictyostelium cofilin and actin. The filaments of the fusion protein were functionally similar to actin filaments bound with cofilin in that they did not bind rhodamine-phalloidin, had quenched fluorescence of pyrene attached to Cys374 and showed enhanced susce...

Distribution among tissues and intracellular localization of cofilin, a 21kDa actin-binding protein.

Cofilin, a 21kDa actin-binding protein, binds to F-actin in a 1:1 molar ratio of cofilin to actin molecule (Nishida, E., S. Maekawa, and H. Sakai, Biochemistry, 23, 5307-5313, 1984) and is capable of controlling actin polymerization and depolymerization in vitro in a pH-sensitive manner (Yonezawa, N., E. Nishida, and H. Sakai, J. Biol. Chem., 260, 14410-14412, 1985). In this study, immunoblot a...

Actin, cofilin and cognition.

1. Nomura, M. & Li, E. Nature 393, 786–790 (1998). 2. Waldrip, W. R. et al. Cell 92, 797–808 (1998). 3. Sirard, C. et al. Genes Dev. 12, 107–119 (1998). 4. Winnier, G. M., Blessing, M., Labosky, P. A. & Hogan, B. L. M. Genes Dev. 9, 2105–2116 (1995). 5. Matzuk, M. M. et al. Nature 374, 354–356 (1995). 6. Conlon, F. L. et al. Development 120, 1919–1928 (1994). 7. Green, J. B., New, H. V. & Smith...

Structural conservation between the actin monomer-binding sites of twinfilin and actin-depolymerizing factor (ADF)/cofilin.

Twinfilin is an evolutionarily conserved actin monomer-binding protein that regulates cytoskeletal dynamics in organisms from yeast to mammals. It is composed of two actin-depolymerization factor homology (ADF-H) domains that show approximately 20% sequence identity to ADF/cofilin proteins. In contrast to ADF/cofilins, which bind both G-actin and F-actin and promote filament depolymerization, t...

Actin Remodeling Protein Cofilin Actin Network Organization through the Expression of Rotavirus NSP4 Alters the