Naringin Attenuates the Fibrosis of Transforming Growth Factor-Beta 1 Induced Human Embryonic Lung Fibroblasts through Nuclear Factor Kappa B Pathway

To investigate the effect of naringin on fibrosis transforming growth factor-beta 1 induced human embryonic lung fibroblasts MRC-5 and its potential mechanism. cells were treated with different concentrations naringin, 5 ng/ml 2 μmol/l nuclear factor kappa B activator phorbol myristate acetate. The experiment was divided into blank group, 1+naringin group 1+naringin+phorbol acetate group. 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide, flow cytometry scratch experiments used to analyze cell viability, cycle distribution migration ability. Western blot examine protein levels. With increase concentration, viability gradually decreased. Compared cycle, rate, alpha-smooth muscle actin, collagen I alpha 1, fibronectin, phosphorylatednuclear p65 phosphorylated-nuclear factor-kappa inhibitor expression in increased. Naringin inhibited factorbeta proliferation, through inactivating pathway. Besides, could reverse naringinmediated inhibition compared reduced 1-induced by inhibiting