Multi level statistical optimization of l-asparaginase from Bacillus subtilis VUVD001
نویسندگان
چکیده
منابع مشابه
Production Optimization of Extracellular L-asparaginase through Solid- State Fermentation by Isolated Bacillus Subtilis
Susmita Shukla and S.K. Mandal Department of Biochemical Engineering and Food Technology, Harcourt Butler Technological Institute, Kanpur-208002, India. E-mail: [email protected] ABSTRACT: L-asparaginase has been used as anti-tumor agent for the treatment of acute lymphoblastic leukemia and food processing aid to reduce the formation of cancer causing acrylamide. Extracellular Lasp...
متن کاملBacillus subtilis 168 contains two differentially regulated genes encoding L-asparaginase.
Expression of the two Bacillus subtilis genes encoding L-asparaginase is controlled by independent regulatory factors. The ansZ gene (formerly yccC) was shown by mutational analysis to encode a functional L-asparaginase, the expression of which is activated during nitrogen-limited growth by the TnrA transcription factor. Gel mobility shift and DNase I footprinting experiments indicate that TnrA...
متن کاملEffective treatment for suppression of acrylamide formation in fried potato chips using L-asparaginase from Bacillus subtilis
It has been reported that acrylamide, a potential carcinogen, is formed from the reaction of L-asparagine (L-Asn) and reducing sugars contained in foods during heating processes and free asparagine is a limiting factor for acrylamide formation. It has been reported that potato products such as potato chips, which are made through heating processes, contain high levels of acrylamide. To decrease...
متن کاملProduction Purification and Characterization of Extracellular Anti-leukaemic L-asparaginase from Isolated Bacillus Subtilis Using Solid State Fermentation
Bacterial L-asparaginase has been widely used as therapeutic agent in treatment of various lymphoblastic leukemia and food processing aid to reduce the formation of cancer causing acrylamide. The present work deals with production and purification of extracellular L-asparaginase from soil isolates using solid state fermentation. The isolate was characterized by big chemical test and identified ...
متن کاملCloning, nucleotide sequence, and expression of the Bacillus subtilis ans operon, which codes for L-asparaginase and L-aspartase.
L-Aspartase was purified from Bacillus subtilis, its N-terminal amino acid sequence was determined to construct a probe for the aspartase gene, and the gene (termed ansB) was cloned and sequenced. A second gene (termed ansA) was found upstream of the ansB gene and coded for L-asparaginase. These two genes were in an operon designated the ans operon, which is 80% cotransformed with the previousl...
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ژورنال
عنوان ژورنال: 3 Biotech
سال: 2017
ISSN: 2190-572X,2190-5738
DOI: 10.1007/s13205-017-1020-2