Mouse testicular protein variants, including tubulin, detected by isoelectrofocusing two-dimensional gel electrophoresis

Two-dimensional Gel Electrophoresis

Identification by Two-Dimensional Gel Electrophoresis

We have previously identified proteins in fractions of culture filtrate ofMycobacterium tuberculosis with the capacity to induce cytokine production in monocytes, by using a technique we defined as "monocyte Western blotting" (immunoblotting). In this series of experiments, we have extended this technique to two-dimensional gel electrophoresis and have identified a novel 58-kDa protein ofM. tub...

Immunogens of Brucella Abortus S19 Identified By Two-Dimensional Gel Electrophoresis and Immunoblotting

Background: Lipopolysaccharides (LPSs) and several antigenic proteins of Brucella have been considered for preparation of diagnostic reagents and subunit vaccines. The objective of this study was to identify and compare immunogens of B. abortus S19 which induce humoral immune responses in human, goat and rabbit. Material and Methods: The bacterial whole cell extract was prepared in extraction b...

Two-Dimensional Gel Electrophoresis (2-DE)

Two-dimensional gel electrophoresis (2-DE) is able to separate hundreds to thousands of proteins or polypeptides by coupling IsoElectric Focusing (IEF) in first dimension and Sodium Dodecyl Sulphate PolyAcrylamide-Gel Electrophoresis (SDS-PAGE) in second dimension. This particular configuration is called classical 2-DE: IEF separates proteins in function of their isoelectric point (pI) and SDS-...

Two-dimensional gel electrophoresis: an overview