Mouse oocyte vitrification: the effects of two methods on maturing germinal vesicle breakdown oocytes
نویسندگان
چکیده
منابع مشابه
Germinal vesicle breakdown in the mouse oocyte.
Germinal vesicle breakdown in mouse oocytes in vivo and in vitro has been examined by electron microscopy. In vitro oocytes were studied immediately after release from follicles and at various times (05-11 h) in culture. Approximately 30 min after oocyte release, chromatin condensation begins along the convoluted nuclear envelope (NE). Chromatin granules are common in all condensing chromosomes...
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Mouse oocytes were cultured in the presence of dibutyryl cyclic AMP (dbcAMP) and various agents that affect cytoplasmic calcium concentrations. Treatment that inhibited calcium uptake potentiated the inhibitory effect of dbcAMP and treatments which stimulated cellular calcium uptake overcame the effect of dbcAMP. Elevated extracellular calcium (greater than 10 mM) significantly decreased the in...
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15 صفحه اولthe effects of cumulus cells on in vitro maturation of mouse germinal vesicle stage oocytes
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متن کاملGlycine increases preimplantation development of mouse oocytes following vitrification at the germinal vesicle stage
Ice-free cryopreservation, referred to as vitrification, is receiving increased attention in the human and animal assisted reproduction. However, it introduces the detrimental osmotic stress by adding and removing high contents of cryoprotectants. In this study, we evaluated the effects of normalizing cell volume regulation by adding glycine, an organic osmolyte, during vitrification of mouse g...
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ژورنال
عنوان ژورنال: Journal of Assisted Reproduction and Genetics
سال: 2010
ISSN: 1058-0468,1573-7330
DOI: 10.1007/s10815-010-9411-x