Molecular study and DNA sequence analysis of Theileria annulata in cattle in Al-Hilla, Iraq

The current work was conducted to unveil the situation for infection by Theileria annulata in cattle Al-Hilla City, Iraq. A total of 225 blood samples (200 from suspected infected animals and 25 clinically healthy as a control group) were collected. These subjected direct slide-smearing detection using microscope DNA sequencing, targeting cytochrome b (Cyt b) gene 10 polymerase chain reaction (PCR) products. thin smear findings 200 cases revealed that 63 (31.5%) with spp., while 115 (57.5%) had no but other parasites; however, only 22 (11%) showed presence any parasites. Unsurprisingly, group demonstrated parasite. Moreover, sequencing spp. belonged T. species, these sequences nucleotide-based similar Gene-Bank isolates Tunisia (ON035604, ON035605, ON035606, ON035607, ON035608, ON035609, ON035610, ON035611, ON035612, ON035613). present study outcomes indicate theileriosis is dominant parasitic City highly caused annulata.