Molecular and Immunological Characterization of Profilin from Mugwort Pollen
نویسندگان
چکیده
منابع مشابه
Molecular and Cellular Characterization of the Tomato Pollen Profilin, LePro1
Profilin is an actin-binding protein involved in the dynamic turnover and restructuring of the actin cytoskeleton in all eukaryotic cells. We previously cloned a profilin gene, designated as LePro1 from tomato pollen. To understand its biological role, in the present study, we investigated the temporal and spatial expression of LePro1 during pollen development and found that the transcript was ...
متن کاملCharacterization of profilin and polcalcin panallergens from ash pollen.
BACKGROUND Ash (Fraxinus excelsior) is an important source of allergenic pollen in temperate areas of Europe. Profilin and polcalcin are 2 important panallergens involved in cross-reactivity between different sources. OBJECTIVE To clone and produce Fra e 2 (profilin) and Fra e 3 (polcalcin) as recombinant proteins and evaluate their immunological properties using the natural forms obtained fr...
متن کاملPCR-based cloning and immunological characterization of Parietaria judaica pollen profilin.
Profilin has been described as an allergen present in pollen of trees, grasses and weeds. Since Parietaria judaica profilin has a molecular mass similar to other Parietaria allergens (Par j 1 and Par j 2) in the 14-10 kDa range, it is difficult to assess the prevalence of profilin by immunoblotting or to obtain sufficient amounts of purified native profilin for investigation and diagnosis. The ...
متن کاملProduction and Characterization of Monoclonal Antibody against Saffron Pollen Profilin, Cro s 2
Background: Allergy to Saffron (Crocus sativus) pollen has been described in people involved in processing of saffron flower stamens. Profilins have been identified as a pan-allergen in different plant pollens and foods. This molecule is an actin-binding pro-tein with a molecular weight of 12-16 kDa found in eukaryotic species. Objective: The aim of this study was to generate monoclonal antibod...
متن کاملIdentification and characterization of a pan-allergen profilin, a major allergen from Caryota mitis pollen.
BACKGROUND Caryota mitis is a common plant in tropical and subtropical areas. It produces larger amount of pollen, which has great potential for allergenicity in the pollination season. OBJECTIVE This study aims to identify the components of Caryota mitis pollen contributing to human allergic diseases. METHODS The sera from 20 patients with a positive skin prick test to Caryota mitis pollen...
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ژورنال
عنوان ژورنال: Biological Chemistry
سال: 2002
ISSN: 1431-6730
DOI: 10.1515/bc.2002.199