Minimal Nutrient Requirements for Culture of One-Cell Rabbit Embryos
نویسندگان
چکیده
منابع مشابه
Development of one-cell porcine embryos in two culture systems.
The effect of culture system and protein supplementation on the development of one-cell porcine embryos was investigated. One hundred and sixty-four one-cell embryos were collected at slaughter from 19 handmated gifts. Culture systems evaluated were microdrops of Whitten's medium (WM) under paraffin oil (M) or tubes with 4.0 ml of WM (T). Also tested was T with a 2.0 ml paraffin oil overlay (O)...
متن کاملImproved development of rabbit one-cell embryos to the hatching blastocyst stage by culture in a defined, protein-free culture medium.
In Exp. 1, Medium 199 and Medium RD (RPMI-1640 and Dulbecco's MEM, 1:1 v/v) were compared in a 2 x 2 factorial design by supplementing each with 15 mg bovine serum albumin (BSA)/ml of 1 mg polyvinyl alcohol (PVA)/ml. All media contained 5 micrograms insulin/ml, 5 micrograms transferrin/ml, 5 ng selenium/ml (ITS), and 10 ng epidermal growth factor (EGF)/ml. One-cell embryos were cultured at 39 d...
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BACKGROUND A comparison has been made of the development of mouse zygotes in either one-step or two-step culture systems. METHODS Embryo culture, blastocyst cell counts and embryo transfer were done. RESULTS No significant differences were observed in the proportions of blastocysts, rates of hatching, numbers of cells in the inner cell mass (ICM) and trophectoderm (TE) that developed in pro...
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Background and Aims: The protozoan parasites of the genus Leishmania are the causative agents of various clinical diseases. Different methods of cultivation of Leishmania parasites are available. In the present study, the efficacy of the LB broth with rabbit lyophilized anti-sheep red blood cell haemolysin was evaluated in the cultivation of promastigotes of Leishmania major. Materials and Met...
متن کاملMinimal enteral nutrient requirements for intestinal growth in neonatal piglets: how much is enough?
BACKGROUND Parenterally nourished preterm infants commonly receive minimal enteral feedings, the aim being to enhance intestinal function. Whether this regimen increases intestinal growth has not been established. OBJECTIVE Our objective was to determine the minimal enteral nutrient intakes necessary to stimulate and to normalize neonatal intestinal growth. METHODS Intestinal growth and cel...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 1987
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod37.4.775