Microsatellite Signature of Reference Genome Sequence of SARS-CoV-2 and 32 Species of Coronaviridae Family

Background: Simple sequence repeats (SSRs) are 1 - 6 bp repeat motif sequences present across both prokaryotic and eukaryotic genomes with various clinical implications besides being tools for conservation evolutionary studies. Objectives: Analysis of 33 Coronavirus genomes, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), incidence, distribution, complexity SSRs patterns to understand their role in host divergence evolution. Methods: Full-length genome were extracted from National Center Biotechnology Information (NCBI). Extraction microsatellites was done using imperfect microsatellite extractor (IMEx) “Advanced Mode”. Sequences aligned MAFFT v6.861b the maximum likelihood tree inferred RAxML v8.1.20 GTR + GAMMA+I model default specifications. Results: A total 3,442 136 complex (cSSRs) studied genomes. SSR incidence ranged 82 (CV09) 144 (CV60). cSSR (CV42, CV43, CV53) 11 (CV32). CV61 (SARS-CoV-2) had 107 SSRs. Di-nucleotide motifs most prevalent followed by tri- mono-nucleotide motifs. TG/GT represented di-nucleotide motif, CA/AC. In tri-nucleotide SSRs, ACA/TGT CAA/GTT, whereas T observed nucleotide, A. About 94% localized coding region. Twenty species, exhibit exclusively A/T region, which clustered phylogenetic analysis. The similarity assessed through heat map analysis revealed similar expectedly placed proximity on tree. Conclusions: Mono-nucleotide exclusivity region signature can be a possible basis predicting evolution viruses terms range.