Mechanism of Reaction of Myeloperoxidase with Nitrite
نویسندگان
چکیده
منابع مشابه
The Reaction of Hemoglobin with Nitrite
Although nitrite has been extensively used as an agent for the formation of methemoglobin, the quantitative relations of the reaction appear to be uncertain; three widely different values have been reported for the amount of methemoglobin formed to nitrite utilized. The first statement of a quantitative relationship was made by Barcroft and Miiller (1) in 1911. In a preliminary report containin...
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A simple, sensitive and rapid method for determination of trace amounts of nitrite ion in real samples is reported. The reaction is initiated by adding known volumes of a nitrite ion solution of identified concentration to acidic solutions of thionine which causes decolorizaiton of reagent with time. The absorbance changes is then monitored spectrophotometrically at l...
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A relatively pure and stable compound III of bovine spleen myeloperoxidase was prepared from native enzyme using the aerobic oxidation of dihydroxyfumarate to generate O2-(.). Spectral scans show well defined peaks at 450 and 625 nm and an isosbestic point between compound III and native enzyme at 440 nm. Compound III decayed to native enzyme without any detectable intermediate. The rate of dec...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2000
ISSN: 0021-9258
DOI: 10.1074/jbc.m000181200