Loop-mediated Isothermal Amplification Assay to Rapidly Detect Wheat Streak Mosaic Virus in Quarantined Plants
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چکیده
منابع مشابه
Loop-mediated Isothermal Amplification Assay to Rapidly Detect Wheat Streak Mosaic Virus in Quarantined Plants.
We developed a loop-mediated isothermal amplification (LAMP) method to rapidly diagnose Wheat streak mosaic virus (WSMV) during quarantine inspections of imported wheat, corn, oats, and millet. The LAMP method was developed as a plant quarantine inspection method for the first time, and its simplicity, quickness, specificity and sensitivity were verified compared to current reverse transcriptio...
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A differential detection method for three wheat viruses: Wheat yellow mosaic virus (WYMV), Japanese soil-borne mosaic virus (JSBWMV) and Chinese wheat mosaic virus (CWMV) using reverse transcription loop-mediated isothermal amplification (RT-LAMP) reaction was developed. All three primer sets, which were designed from the genome sequences of WYMV, JSBWMV and CWMV respectively, worked most effic...
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Diagnostic methods for detecting and quantifying HIV RNA have been improving, but efficient methods for point-of-care analysis are still needed, particularly for applications in resource-limited settings. Detection based on reverse-transcription loop-mediated isothermal amplification (RT-LAMP) is particularly useful for this, because when combined with fluorescence-based DNA detection, RT-LAMP ...
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مقدمه:سالمونلاها از مهمترین باکتری های بیماری و عامل تیفوئید، باکتریمی، آنتروکولیت و سالمونلوز است که از عفونت های مشترک انسان و حیوانات می باشد. بیماریهای ناشی از این عامل یک معضل بزرگ بهداشتی در جهان به خصوص در کشورهای در حال توسعه از جمله کشور ما می باشد. بنابراین تشخیص سریع، دقیق و به موقع آن برای جلوگیری از اپیدمی و عوارض مخرب این باکتری ضروری به نظر می رسد. برای تشخیص سالمونلا روش های مخت...
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ژورنال
عنوان ژورنال: The Plant Pathology Journal
سال: 2015
ISSN: 1598-2254
DOI: 10.5423/ppj.nt.06.2015.0110