Long noncoding RNA LINC00958 suppresses apoptosis and radiosensitivity of colorectal cancer through targeting miR-422a

Abstract Background Long noncoding RNAs (lncRNAs) have been elucidated to participate in the development and progression of various cancers. In this study, we aimed explore underlying functions mechanisms LINC00958 colorectal cancer. Methods expression cancer tissues was examined by qRT-PCR. The correlations between clinical characteristics prognosis were evaluated. biological detected CCK-8, MTT, colony formation flow cytometric analyses. RNA pulldown, RIP luciferase reporter assays used confirm regulatory effects on miR-422a. Rescue experiments performed detect miR-422a roles LINC00958. Results upregulated cell lines. High levels positively associated with T stage predicted poor prognosis. Cell showed that promoted proliferation suppressed apoptosis sensitivity radiotherapy vitro tumor growth vivo. Bioinformatics analysis binding site Mechanistically, demonstrated specifically targeted addition, found MAPK1 directly 3’-UTR MAPK1, thereby inhibiting enhancing radiosensitivity. Furthermore, rescued promoting decreasing Conclusions radiosensitivity targeting miR-422a, which suggests it is a potential biomarker for treatment