Leishmania mexicana recombinant filamentous acid phosphatase as carrier for Toxoplasma gondii surface antigen 1 expression in Leishmania tarentolae
نویسندگان
چکیده
منابع مشابه
Recombinant protein expression in Leishmania tarentolae.
A variety of recombinant protein expression systems have been developed for heterologous genes in both prokaryotic and eukaryotic systems such as bacteria, yeast, mammals, insects, transgenic animals, and plants. Recently Leishmania tarentolae, a trypanosomatid protozoan parasite of the white-spotted wall gecko (Tarentola annularis), has been suggested as candidate for heterologous genes expres...
متن کاملCharacterization of the growth behavior of Leishmania tarentolae: a new expression system for recombinant proteins.
Biotechnological production of recombinant proteins for human therapy requires a cultivation of the host organism in a nutrient medium free of animal substances. Therefore, various nutrient media for the new expression system Leishmania tarentolae were developed and examined according to their cultivation conditions as static suspension culture and agitated culture. Investigations resulted in t...
متن کاملRecombinant MAG1 Protein of Toxoplasma gondii as a Diagnostic Antigen.
The aim of this study was to evaluate the potential diagnostic usefulness of the full-length recombinant Toxoplasma gondii MAG1 protein by determining the levels of specific IgM and IgG antibodies in mouse and human sera obtained from individuals with acute and chronic toxoplasmosis. The obtained results revealed that IgG antibodies against MAG1 are a sensitive and specific marker of T. gondii ...
متن کاملRecombinant scFv Antibodies against P30 Surface Protein of Toxoplasma Gondii
Background & Aims: Toxoplasma gondii is an obligate, intracellular parasite, which is widely spread in the world. The parasite is able to infect all warm-blooded hosts including humans and farm animals. The infection in humans often occurs after the ingestion of raw or undercooked meat containing tissue cysts. Several methods have been applied to detect this parasite in contaminated foods. Reco...
متن کاملThe production of recombinant human laminin-332 in a Leishmania tarentolae expression system.
Laminin (LM)-332 (alpha3beta3gamma2), a large heterotrimeric glycoprotein, is an essential component of epithelial basement membranes that promotes cell adhesion and migration. Here, we expressed human LM-332 using a novel protein expression system based on the trypanosomatid protozoan host Leishmania tarentolae. Plasmids containing cDNA encoding full-length beta3 and gamma2 subunits and trunca...
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ژورنال
عنوان ژورنال: Journal of Parasitic Diseases
سال: 2021
ISSN: 0971-7196,0975-0703
DOI: 10.1007/s12639-021-01413-x