Isolation and Culture of Pulmonary Endothelial Cells
نویسندگان
چکیده
منابع مشابه
Isolation and culture of pulmonary endothelial cells.
Methods for isolation, identification and culture of pulmonary endothelial cells are now routine. In the past, methods of isolation have used proteolytic enzymes to detach cells; thereafter, traditional methods for cell passaging have used trypsin/EDTA mixtures. Cells isolated and passaged using proteolytic enzymes have been useful in establishing the field and in verifying certain endothelial ...
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Endothelial cells provide a useful research model in many areas of vascular biology. Since its first isolation, human umbilical vein endothelial cells (HUVECs) have shown to be convenient, easy to obtain and culture, and thus are the most widely studied endothelial cells. However, for research focused on processes like angiogenesis, permeability or many others, microvascular endothelial cells (...
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ژورنال
عنوان ژورنال: Environmental Health Perspectives
سال: 1984
ISSN: 0091-6765
DOI: 10.2307/3429840