Insoluble derivatives of polynucleotide-phosphorylase
نویسندگان
چکیده
منابع مشابه
Polynucleotide phosphorylase of Micrococcus lysodeiktpcus. III. The apparent arsenolysis of nucleoside diphosphates by polynucleotide phosphorylase.
The enzyme, polynucleotide phosphorylase, catalyzes the polymerization of nucleoside diphosphates to polyribonucleotides with the formation of inorganic orthophosphate (1, 2). The reaction is readily reversible, and the phosphorolysis of polyribonucleotides has been studied extensively (3-6). Several recent reviews (7-9) afford extensive summaries of the literature. In the accompanying paper (l...
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In the fall of 1944, I enrolled at the Hebrew University of Jerusalem. The student body numbered about 700 and the choice of faculties was somewhat limited.My hopewas to study medicine, but the plans to open a medical school were still at the drawing board stage. I therefore chose to study chemistry with biochemistry and bacteriology as minor subjects, which I thought I would need later if I we...
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In this report, we document the presence of polynucleotide phosphorylase (PNPase) in the animal eukaryotes. These proteins contain several domains, including 2 RNase PH domains (PNPase 1 and PNPase 2) which are closely related functionally and in sequence similarity to ribonuclease PH (RPH) protein. Phylogenetic analysis of the gene genealogy of these three domains suggests that PNPase was form...
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The purpose of this paper is to review certain aspects of the mechanism of action of polynucleotide phosphorylase and to present, rather briefly, some recent findings. The discussion will be concerned with studies carried out by S. Ochoa and his associates a t New York University, New York, N. Y., and with work done a t the National Institutes of Health. Reference will also be made to some rece...
متن کاملPhosphorolysis of oligoribonucleotides by polynucleotide phosphorylase.
This reversible reaction has been demonstrated With enzyme preparations from Azotobacter trinelundii (1, 2) and Escherichia coli (3). In the forward direction the enzyme catalyzes the formation of polyribonucleotides similar in structural details to natural RNA1 (5-8). In the reverse reaction polyribonucleotides are phosphorolyzed to yield nucleoside diphosphates. The phosphorolysis of syntheti...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1973
ISSN: 0014-5793
DOI: 10.1016/0014-5793(73)80662-3