In-Gel Imaging of RNA Processing Using Broccoli Reveals Optimal Aptamer Expression Strategies
نویسندگان
چکیده
منابع مشابه
Single-Molecule Imaging of an in Vitro-Evolved RNA Aptamer Reveals Homogeneous Ligand Binding Kinetics
Many studies of RNA folding and catalysis have revealed conformational heterogeneity, metastable folding intermediates, and long-lived states with distinct catalytic activities. We have developed a single-molecule imaging approach for investigating the functional heterogeneity of in vitro-evolved RNA aptamers. Monitoring the association of fluorescently labeled ligands with individual RNA aptam...
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ژورنال
عنوان ژورنال: Chemistry & Biology
سال: 2015
ISSN: 1074-5521
DOI: 10.1016/j.chembiol.2015.04.018