Identification and characterization of a zinc metalloprotease associated with invasion by the fish pathogen Vibrio anguillarum
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چکیده
منابع مشابه
Identification and characterization of a zinc metalloprotease associated with invasion by the fish pathogen Vibrio anguillarum.
An invasiveness-defective mutant of the fish-pathogenic bacterium Vibrio anguillarum was isolated. Compared with the wild type, this mutant had a 1,000-fold higher 50% lethal dose after immersion infection of rainbow trout, Oncorhynchus mykiss, while after intraperitoneal infection, the mutant had only a 10-fold higher 50% lethal dose. In addition, the mutant showed a lower level of protease ac...
متن کاملIdentification and characterization of Epp, the secreted processing protease for the Vibrio anguillarum EmpA metalloprotease.
The zinc metalloprotease EmpA is a virulence factor for the fish pathogen Vibrio anguillarum. Previous studies demonstrated that EmpA is secreted as a 46-kDa proenzyme that is activated extracellularly by the removal of an approximately 10-kDa propeptide. We hypothesized that a specific protease is responsible for processing secreted pro-EmpA into mature EmpA. To identify the protease responsib...
متن کاملMolecular characterization of the TonB2 protein from the fish pathogen Vibrio anguillarum.
In the fish pathogen Vibrio anguillarum the TonB2 protein is essential for the uptake of the indigenous siderophore anguibactin. Here we describe deletion mutants and alanine replacements affecting the final six amino acids of TonB2. Deletions of more than two amino acids of the TonB2 C-terminus abolished ferric-anguibactin transport, whereas replacement of the last three residues resulted in a...
متن کاملIron(III) complexation by Vanchrobactin, a siderophore of the bacterial fish pathogen Vibrio anguillarum.
The bacterial fish pathogen Vibrio anguillarum serotype O2 strain RV22 produces the mono catecholate siderophore Vanchrobactin (Vb) under conditions of iron deficiency. Vb contains two potential bidentate coordination sites: catecholate and salicylate groups. The iron(III) coordination properties of Vb is investigated in aqueous solutions using spectrophotometric and potentiometric methods. The...
متن کاملRole of motility in adherence to and invasion of a fish cell line by Vibrio anguillarum.
To understand further the role of the flagellum of Vibrio anguillarum in virulence, invasive and adhesive properties of isogenic motility mutants were analyzed by using a chinook salmon embryo cell line. Adhesion was unaffected but invasion of the cell line was significantly decreased in nonmotile or partially motile mutants, and the chemotactic mutant was hyperinvasive. These results suggest t...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1990
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.58.11.3731-3736.1990