High throughput RNase protection assay
نویسندگان
چکیده
منابع مشابه
Ribonuclease protection assay streamlined for high throughput with RNA in formamide and single-step rnase inactivation.
The ribonuclease protection assay (RPA) is a powerful technique for measuring gene expression, but it is laborious, especially when large numbers of samples are analyzed. We developed a streamlined RPA protocol that eliminates two of the most tedious steps in the standard procedure. First, the need for re-precipitation of sample RNA before hybridization with labeled probes is eliminated by resu...
متن کاملFluorescence-based, high-throughput DNA polymerase assay.
The commonly used DNA polymerase assay is based on the detection of incorporated radiolabeled nucleotides in a DNA elongation reaction. It is laborious, radioactive, and can be highly variable. Here we report a nonradioactive fluorescence-based assay. The method consists of Cydye-labeled nucleotides, biotinylated primer, and a streptavidin-coated microplate. The assay is found to have sensitivi...
متن کاملAutomated high-throughput sex-typing assay.
Determining the sex of the donor of a human DNA sample in a rapid and accurate manner is of importance in several settings, including basic research, clinical practice, and forensics (1,2). Our group routinely performs high-throughput ge-notyping within large epidemiological studies. It is not uncommon to receive thousands of DNA samples, with the tube identification as the only accompanying in...
متن کاملHigh-throughput Saccharification Assay for Lignocellulosic Materials
Polysaccharides that make up plant lignocellulosic biomass can be broken down to produce a range of sugars that subsequently can be used in establishing a biorefinery. These raw materials would constitute a new industrial platform, which is both sustainable and carbon neutral, to replace the current dependency on fossil fuel. The recalcitrance to deconstruction observed in lignocellulosic mater...
متن کاملKwok, "High-Throughput Genotyping Assay Approaches"
High-throughput genotyping approaches are being developed to meet the demands of pharmacogenomics, where numerous individuals are studied with thousands of single nucleotide polymorphism (SNP) markers. All non-gel-based genotyping approaches achieve allelic discrimination by one of four mechanisms: allele-specific hybridisation, allele-specific primer extension, allele-specific oligonucleotide ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1995
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/23.16.3359