Functional annotation of the human retinal pigment epithelium transcriptome
نویسندگان
چکیده
منابع مشابه
Transcriptome analysis and molecular signature of human retinal pigment epithelium
Retinal pigment epithelium (RPE) is a polarized cell layer critical for photoreceptor function and survival. The unique physiology and relationship to the photoreceptors make the RPE a critical determinant of human vision. Therefore, we performed a global expression profiling of native and cultured human fetal and adult RPE and determined a set of highly expressed 'signature' genes by comparing...
متن کاملTissue culture of human retinal pigment epithelium.
Adult human retinal pigment epithelium has been enzymatically isolated and established In tissue culture. Primary cultures survived three to six months. Occasionally, cell lines spontaneously evolved from primary cultures. Melanin granules decreased with the age of the culture, became less pigmented but retained some characterstics of melanin-producing cells. Lipofuscin granules are present, bu...
متن کاملProtein database, human retinal pigment epithelium.
The retinal pigment epithelium (RPE) is a single cell layer adjacent to the rod and cone photoreceptors that plays key roles in retinal physiology and the biochemistry of vision. RPE cells were isolated from normal adult human donor eyes, subcellular fractions were prepared, and proteins were fractionated by electrophoresis. Following in-gel proteolysis, proteins were identified by peptide sequ...
متن کاملThe interaction of indocyanine green with human retinal pigment epithelium.
PURPOSE This study sought to examine the in vitro interaction of human RPE cells with indocyanine green (ICG). The interaction between ICG and the RPE may have clinical relevance in the interpretation of clinical ICG angiography. METHOD Cultured primary human RPE cells were incubated with ICG. Infrared fluorescence microscopy was used to detect RPE cell ICG fluorescence. The proportions of ce...
متن کاملHistochemical study of retinal photoreceptors development during pre- and postnatal period and their association with retinal pigment epithelium
Objective(s):The aim of this study was to evaluate distribution and changes of glycoconjugates of retinal photoreceptors during both pre- and postnatal development. Materials and Methods: Tissue sections from days 15 to 20 of Wistar rat embryos and 1 to 12 postnatal days of rat newborns including developing eye were prepared for lectinhistochemistry technique. Horseradish peroxidase (HRP)-label...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMC Genomics
سال: 2009
ISSN: 1471-2164
DOI: 10.1186/1471-2164-10-164