منابع مشابه
Facts and Artifacts of Ancient DNA
Tomas Lindahl technical lessons, since initial attempts to recover Imperial Cancer Research Fund mtDNA from that great archaeological find resulted in Clare Hall Laboratories three distinct human sequences, two of which had to South Mimms be ascribed to contamination with modern human DNA. Hertfordshire EN6 3LD (A reliable sequence was subsequently produced by United Kingdom independent confirm...
متن کاملAncient DNA from Human and Animal
Research on ancient DNA (aDNA) has the potential to enable molecular biologists and archeologists to decipher certain aspects of history by direct looking into the past. However, several major problems in this field limit the applicability of aDNA studies, most importantly contamination with modern DNA and postmortem DNA degradation. In this study we extracted and analyzed aDNA obtained from ~3...
متن کاملDNA sequences from multiple amplifications reveal artifacts induced by cytosine deamination in ancient DNA.
We show that DNA molecules amplified by PCR from DNA extracted from animal bones and teeth that vary in age between 25 000 and over 50 000 years carry C-->T and G-->A substitutions. These substitutions can reach high proportions among the molecules amplified and are due to the occurrence of modified deoxycytidine residues in the template DNA. If the template DNA is treated with uracil N-glycosy...
متن کاملFacts and artifacts in the blinking statistics of semiconductor nanocrystals.
Since its initial discovery just over a decade ago, blinking of semiconductor nanocrystals has typically been described in terms of probability distributions for durations of bright, or "on," states and dark, or "off," states. These distributions are obtained by binning photon counts in order to construct a time series for emission intensity and then applying a threshold to distinguish on state...
متن کاملCorrection: Palindromic Sequence Artifacts Generated during Next Generation Sequencing Library Preparation from Historic and Ancient DNA
Degradation-specific processes and variation in laboratory protocols can bias the DNA sequence composition from samples of ancient or historic origin. Here, we identify a novel artifact in sequences from historic samples of Atlantic cod (Gadus morhua), which forms interrupted palindromes consisting of reverse complementary sequence at the 5' and 3'-ends of sequencing reads. The palindromic sequ...
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ژورنال
عنوان ژورنال: Cell
سال: 1997
ISSN: 0092-8674
DOI: 10.1016/s0092-8674(00)80306-2