FACTORS INFLUENCING VARIOLA VIRUS GROWTH ON THE CHORIOALLANTOIC MEMBRANE OF EMBRYONATED EGGS

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Factors influencing variola virus growth on the chorioallantoic membrane of embryonated eggs.

The propagation of variola virus on the chlorioallantoic membrane of embryonated eggs was first reported by Torres and Teixeira (1935). Application of this finding was made by Lazarus et al. (1937), Buddingh (1938) and Markham and Bozalis (1939) who utilized embryonated eggs as a diagnostic medium. Nelson (1940, 1943), described the development and stability of variola virus in egg passage but ...

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The Stability of Variola Virus Propagated in Embryonated Eggs

After 24 transfers in embryonated eggs a strain of variola virus (Chinese) was established in the testis of the rabbit and maintained for 11 passages at intervals of 7 days. Residence in the rabbit testis was not accompanied by any significant alteration in the species identity of the virus. A second strain of variola virus (Minnesota) was transferred 180 times in embryonated eggs with no appar...

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Effect of in vitro adaptation of Marek's disease virus on pock induction on the chorioallantoic membrane of embryonated chicken eggs.

Cell-associated preparations of several isolates of Marek's disease virus produced more pocks on the chorioallantoic membrane of embryonated chicken eggs than plaques in duck embryo fibroblasts, thus indicating that lesion response in eggs was more sensitive than cytopathic response in duck embryo fibroblasts for assaying low-passage Marek's disease virus. Adaptation of the virus to cell cultur...

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Survival of variola virus in chorioallantoic membrane preparations.

The transmission of smallpox by contaminated bed clothes (Stallybrass, 1931) and by dust from the floor of a patient's room (Downie and Dumbell, 1947) is evidence of the stability of variola virus under natural conditions. Experimental proof of the survival of variola virus in exudates and crusts from humans was reported by Buddingh (1938) and North et al., (1944). The former investigator noted...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1958

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.75.6.707-712.1958