Expression pattern of alkaline phosphatase in Dictyostelium
نویسندگان
چکیده
منابع مشابه
Expression pattern of alkaline phosphatase in Dictyostelium
We used two different methods to study the expression pattern of alkaline phosphatase (alp) in Dictyostelium. In situ staining of the endogenous enzyme activity at different stages of development showed that the enzyme was active early in the aggregation stage and localized to the area where the tip of the first finger was initiated. The activity was localized to the anterior region of developi...
متن کاملoptimization of alkaline phosphatase gene expression in e. coli
alkaline phosphatase is an enzyme with widespread use in research and industry such as protein labeling, dephosphorylation of nucleic acids, and enzyme based biosensors. in the present study, alkaline phosphatase gene was inserted into the pet15b vector. the recombinant dna was then expressed using iptg as an inducer. the expression of this enzyme was optimized by changing expression conditions...
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In order to analyze the expression pattern of the 5'-nucleotidase (5nt) gene in Dictyostelium, we made a fusion construct in which the 5nt promoter directed the expression of beta-galactosidase gene. The reporter gene was not active in vegetative amoebae but was expressed during the aggregation stage. At the slug stage, 5nt was highly expressed in pstAB cells. As the slug moved along the substr...
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Background: The present study was carried out on the human population of Kashmir valley to evaluate the status of the biochemical parameters of infected population. Materials and Methods: Blood samples were collected from 514 individuals, 298 (57.97%) males and 216 (42.02%) females, 187 (36.38%) were found sero-positive for human toxocariasis. Results: Alkaline phosphatase level was found highe...
متن کاملOptimization of alkaline phosphatase gene expression in E. coli
Alkaline phosphatase is an enzyme with widespread use in research and industry such as protein labeling, dephosphorylation of nucleic acids, and enzyme based biosensors. In the present study, alkaline phosphatase gene was inserted into the pET15b vector. The recombinant DNA was then expressed using IPTG as an inducer. The expression of this enzyme was optimized by changing expression conditions...
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ژورنال
عنوان ژورنال: Mechanisms of Development
سال: 2002
ISSN: 0925-4773
DOI: 10.1016/s0925-4773(02)00214-9