Evaluation of chromogenic culture media for rapid identification of microorganisms isolated from cows with clinical and subclinical mastitis

This study aimed to evaluate the diagnostic performance (specificity, Sp; sensitivity, Se; accuracy; positive predictive value; negative and Cohen's kappa coefficient, ?, of agreement) chromogenic culture media for rapid identification microorganisms isolated from cows with clinical (CM) subclinical mastitis (SCM). For this, 2 experiments were carried out: evaluation (1) biplate, (2) triplate mastitis-causing microorganisms. performance, by MALDI-TOF mass spectrometry was considered standard methodology. In experiment 1, 476 milk samples collected CM 660 SCM evaluated inoculation in selective (CHROMagar) gram-positive bacteria another gram-negative bacteria. 2, 500 (Smartcolor2, Onfarm), Streptococcus Strep-like organisms, Staphylococcus, 1 samples, use biplates showed Se that ranged 0.56 (0.32–0.81; Staphylococcus aureus) 0.90 (0.80–0.99 uberis), Sp varied 0.94 (0.92–0.96; Strep. uberis) 1.00 (Prototheca spp. or yeast), ? 0.47 (0.26–0.67; Staph. 0.84 (0.78–0.9; Escherichia coli). The 0.50 (0.15–0.85; E. coli) (0.87–1.00; aureus), 0.95 (0.93–0.97; 0.99 (0.98–1.00; aureus Agalactiae dysgalactiae), 0.18 (0.00–0.40; 0.88 (0.80–0.95; aureus). 0.09 (0.00–0.26; Serratia spp.) (0.85–1.00; Klebsiella Enterobacter spp.), agalactiae dysgalactiae) (Serratia 0.07 (0.00–0.24; 0.85 (0.75–0.94; spp.). 0.25 (0.10–0.40; Lactococcus (Strep. 0.92 (0.90–0.94; 0.28 (0.00–0.72; 0.72 (0.60–0.82; Our results suggest accuracy biplate varies according pathogen, may be useful decision-making on treatment protocols main microorganisms, but their implementation control measures will depend each farm specific needs.