Erythrocyte Senescent Markers by Flow Cytometry

Assessment of erythrocyte shape by flow cytometry techniques.

BACKGROUND Red blood cell (RBC) rheology is altered in different diseases, including acute conditions such as patients in intensive care units (ICU) with sepsis or with an inflammatory reaction due to postoperative states or intracerebral haemorrhage, or chronic conditions such as diabetes mellitus or terminal renal failure. Several techniques are available to assess alterations in RBC rheology...

Fluorescent erythrocyte ghosts as standards for quantitative flow cytometry.

We report here a quick and inexpensive method for preparing standards of known fluorochrome content for calibration and quantitation of flow cytometry fluorescence signals. Erythrocyte ghosts prepared by hypotonic lysis are filled with solutions containing fluorescently labeled dextran. Standards prepared by this technique have a narrow range of fluorescence and a linear response of fluorescenc...

Genetic Variation of Lime (Citrus sp.) Accessions Using Flow Cytometry Technique, Morphological Characteristics and Molecular Markers

Because of sexual propagation of Mexican lime in southern regions of Iran, there are many lime accessions (lime biotypes) in Mexican lime gardens in which appear some variation in fruit and tree shape. However, most of these accessions are susceptible to witches broom disease of lime (WBDL). Persian lime (C. latifolia Tanaka) is a triploid WBDL-tolerant species. Considerable number of un-descri...

Functional Assays by Flow Cytometry

Cell function assays have been redefined over the past several years, largely because of technologies such as flow cytometry. The major difference between other techniques and those proposed using flow cytometry is the ability to analyze viable, single cells in a rapid and quantitative fashion. One of the advantages of flow cytometry methods lies in the ability to obtain population information....

Phosphoproteins by Flow Cytometry Surface Markers and Intracellular Coordinate Analysis of Murine Immune Cell