Erratum to: Abstract P-1003--An Approach Towards Generating Selectable Marker Free, Transgenic, Insecticidal Chickpea by Means of Plumular Meristem Transformation
نویسندگان
چکیده
منابع مشابه
A Site-Specific Recombinase-Based Method to Produce Antibiotic Selectable Marker Free Transgenic Cattle
Antibiotic selectable marker genes have been widely used to generate transgenic animals. Once transgenic animals have been obtained, the selectable marker is no longer necessary but raises public concerns regarding biological safety. The aim of this study was to prepare competent antibiotic selectable marker free transgenic cells for somatic cell nuclear transfer (SCNT). PhiC31 intergrase was u...
متن کاملImproved site-specific recombinase-based method to produce selectable marker- and vector-backbone-free transgenic cells
PhiC31 integrase-mediated gene delivery has been extensively used in gene therapy and animal transgenesis. However, random integration events are observed in phiC31-mediated integration in different types of mammalian cells; as a result, the efficiencies of pseudo attP site integration and evaluation of site-specific integration are compromised. To improve this system, we used an attB-TK fusion...
متن کاملSelectable Marker Gene Removal and Expression of Transgene by Inducible Promoter Containing FFDD Cis-Acting elements in Transgenic plants
Abstract Background: Selectable marker gene (SMG) systems are critical for generation of transgenic crops. Transgenic crop production Background: Selectable marker gene (SMG) systems are critical for generation of transgenic crops. Transgenic crop production without using SMG is not economically feasible. However, SMGs are non-essential once an intact transgenic plant has been established. Eli...
متن کاملevaluation of spirulina platensis resistance to different antibiotics to find a selectable marker for genetic transformation
background spirulina is one of the most profitable known microalgae in the world, which is used as food and superfood. in the other hand, spirulina is a useful source of healthy components. it seems that the spirulina is transformable so that the introduction of a selectable marker is needed. objectives the purpose of this study was to determine a suitable selection marker for spirulina platens...
متن کاملA rapid and robust selection procedure for generating drug-selectable marker-free recombinant malaria parasites
Experimental genetics have been widely used to explore the biology of the malaria parasites. The rodent parasites Plasmodium berghei and less frequently P. yoelii are commonly utilised, as their complete life cycle can be reproduced in the laboratory and because they are genetically tractable via homologous recombination. However, due to the limited number of drug-selectable markers, multiple m...
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ژورنال
عنوان ژورنال: In Vitro Cellular & Developmental Biology - Animal
سال: 2017
ISSN: 1071-2690,1543-706X
DOI: 10.1007/s11626-017-0193-7