Epidemiological survey and screening strategy for dengue virus in blood donors from Yunnan Province

Abstract Background Dengue virus (DENV) infection is increasingly common in southern China and can be transmitted through blood transfusion but not currently part of donor screening throughout the region. We assessed DENV prevalence among donors at Xishuangbanna Blood Center, Yunnan, to support development strategies. Methods samples were collected randomly between June 2019 August 2019. These screened for anti-DENV IgG IgM using enzyme-linked immunosorbent assay (ELISA). Then, all reactive some randomly-chosen non-reactive used detect RNAs real-time polymerase-chain-reaction (RT-PCR) assays. After RT-PCR, further tested soluble nonstructural protein 1 (NS1) colloidal gold method. Donors demographics also assessed. Results Over study period, 2254 by ELISA. This revealed 598 and/or samples, a serological 26.53%. Of these, 26 RT-PCR positive NS1 positive. Significant differences noted occupation ( P = 0.001 ), education < ethnicity 0.026 ). Conclusion The Center was higher than most other centers that have implemented screening. Our provides first-hand data about allows strategy clinical use.