Enhanced Ribozyme‐Catalyzed Recombination and Oligonucleotide Assembly in Peptide‐RNA Condensates

نویسندگان

چکیده

The ability of RNA to catalyze ligation is critical its central role in many prebiotic model scenarios, particular the copying information during self-replication. Prebiotically plausible ribozymes formed from short oligonucleotides can reversible cleavage and reactions, but harsh conditions or unusual scenarios are often required promote folding drive reaction equilibrium towards ligation. Here, we demonstrate that ribozyme activity greatly enhanced by charge-mediated phase separation with poly-L-lysine, which shifts solution peptide-RNA coaggregates coacervates. This compartmentalization enables robust isothermal assembly over a broad range conditions, be leveraged assemble long complex RNAs fragments under mild absence exogenous activation chemistry, bridging gap between pools oligomers functional RNAs.

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ژورنال

عنوان ژورنال: Angewandte Chemie

سال: 2021

ISSN: ['1521-3773', '1433-7851', '0570-0833']

DOI: https://doi.org/10.1002/ange.202109267