Engineering hemoglobin to enable homogenous PEGylation without modifying protein functionality

Versatile Route to Synthesize Heterobifunctional Poly(ethylene glycol) of Variable Functionality for Subsequent Pegylation

Pegylation using heterotelechelic poly(ethylene glycol) (PEG) offers many possibilities to create high-performance molecules and materials. A versatile route is proposed to synthesize heterobifunctional PEG containing diverse combinations of azide, amine, thioacetate, thiol, pyridyl disulfide, as well as activated hydroxyl end groups. Asymmetric activation of one hydroxyl end group enables the ...

Hemoglobin Strain Field Waves and Allometric Functionality

Hemoglobin (Hgb) forms tetramers (dimerized dimers), which enhance its globular stability and may also facilitate small gas molecule transport, as shown by recent allatom Newtonian solvated simulations. Hydropathic bioinformatic scaling reveals many wave-like features of strained Hgb structures at the coarse-grained amino acid level, while distinguishing between these features thermodynamically...

Trader Cooperation to Enable Object Sharing among Users of Homogenous Distributed Systems*

This paper demonstrates that it is possible to build a trading remote distributed systems. This service is an extension to an maximum possible autonomy and flexibility to users yet at the same objects (resource, service) within a local distributed computer attribute names, name domains, the operations of object export, achieving object sharing among a number of users of independent which form t...

Chemistry for peptide and protein PEGylation.

Poly(ethylene glycol) (PEG) is a highly investigated polymer for the covalent modification of biological macromolecules and surfaces for many pharmaceutical and biotechnical applications. In the modification of biological macromolecules, peptides and proteins are of extreme importance. Reasons for PEGylation (i.e. the covalent attachment of PEG) of peptides and proteins are numerous and include...

Monitoring Protein PEGylation with Ion Exchange Chromatography