Efficient conjugation of anti-HBsAg antibody to modified core-shell magnetic nanoparticles (Fe₃O₄@SiO₂/NH₂)

Introduction: Further development of magnetic-based detection techniques could be significant use in increasing the sensitivity and quantification hepatitis B virus (HBV) infection. The present work addresses fabrication characterization a new bio-nano composite based on immobilization goat anti-HBsAg antibody modified core-shell magnetic nanoparticles (NPs) by (3-aminopropyl) triethoxysilane (APTES), named Fe3O4@SiO2/NH2, NPs chitosan (Fe3O4@CS). Methods: At first step, Fe3O4 was with silica APTES (Fe3O4@SiO2/NH2) (Fe3O4@CS) separately. activated two different protocols: Sodium periodate EDC-NHS. Then resulted composites were conjugated IgG. An external magnet collected Bio-super (BSMNPs) remained solution analyzed Bradford method to check amount attached surface BSMNPs. Results: findings indicated that activation antibodies sodium 15-17 µg immobilized 1 mg super (SMNPs). However, EDC-NHS method, 8-10 SMNPs. resulting bio-magnetic applied for interaction HBsAg target using enzyme-linked immunosorbent assay (ELISA). About antigen SMNPs, which demonstrated fabricated materials are applicable scope HBsAg. Conclusion: In study, we developed antibody-conjugated an efficient conjugation strategy. results binding capacity Fe3O4@SiO2/NH2 comparable commercially available products. Our designed conjugating nanoparticle opens way produce high NPs.